PARP1 and MGMT protein interaction in response to DNA damage induction

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The invention is related to the protein interaction between PARP1 and MGMT that has not been shown before. These proteins belong to two DNA repair pathways: PARP1 repairs DNA damage as part of base excision repair mechanism, and MGMT is suicide protein repairing a single type of DNA lesion, O6-methylguanine. In untreated Ewing sarcoma cells, the levels of these proteins in complexes are very low or undetectable, however, when cells were treated with DNA methylating agent temozolomide, the interaction between these proteins has increased. This phenomenon has been experimentally confirmed in our lab in several ways: co-immunoprecipitation, co-localization by immunostaining, and by microscale thermophoresis analysis.

Small Molecule
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